Potentiometric Determination of Enrofloxacin Using PVC and Coated Graphite Sensors

A novel approach for the determination of Enrofloxacin (ENR) in its pure form and pharmaceutical formulation is presented. Two ion selective electrode sensors were designed for determination of enrofloxacin namely; ENR-PVC sensor and ENR-Coated graphite sensor. The sensors are based on the ion association complexes of ENR cation with sodium tetra phenyl borate (ENR-TPB) counter anions as ion exchange sites using dioctylphathalate (DOP) as plasticizer. The sensors are used for determination of ENR, in its pure form and in pharmaceutical preparation. Validation of the method shows suitability of the proposed sensors for use in the quality control assessment of ENR and for its routine analysis. The results obtained were statistically compared to reference method and there were no significant difference between the proposed methods and the reference methods regarding the accuracy and precision. The proposed method was validated according to ICH guidelines and the results were satisfactory. Conclusion: The method was found to be accurate, precise time and cost saving unlike HPLC procedures and successfully applied for determination of enrofloxacin in pure and dosage form.

Keywords: Enrofloxacin; PVC; Coated graphite; Potentiometry.